【Applied Biosystems】ABI Prism® 7000 Fluorescence Quantitative PCR Instrument

ABI Prism® 7000 Fluorescence Quantitative PCR Instrument is faster and easier to operate

• Multicolor fluorescence detection

• Real-time data monitoring

• Analysis of melting curve real-time display

• Simplified allele determination (for SNPs analysis)

• Negative/positive results are automatically determined

• Space-saving design

The ABI 7000 fluorescence quantitative PCR instrument uses precise optical principles and complex multi-component algorithms to accurately detect and process exponentially amplified PCR data, yielding high-precision and reproducible Ct values (there is a linear relationship between the Ct value and the initial copy number).

The installation index of the ABI 7000 type fluorescent quantitative PCR instrument: using the TaqMan® RNase P Instrument Detection Plate, the ABI 7000 fluorescence quantitative PCR instrument can distinguish the template copy number between 5,000 and 10,000 with a confidence of up to 99.7%.

During the reaction, the 96 sample wells of the ABI 7000 Sequence Detection System were excited by a tungsten halogen lamp, and the generated fluorescence was passed through an optical filter to a CCD camera. The filter wheel can effectively distinguish many fluorescent dyes including FAMTM/SYBR® Green I, VICTM/JOETM, TAMRATM, and ROXTM. Multicolor fluorescence detection technology enables multiple quantification, SNPs genotyping and anion/negative analysis with internal positive controls.

ABI Prism® 7000 sequence detection system is compatible with two chemical reagents:

• The 5’’ nuclease assay using the TaqMan® probe provides superior sensitivity and specificity, and enables multiplex reactions.

• SYBR® Green I dye is an ideal chemical reagent for target gene identification or a small amount of reaction.

Regardless of the chemical reagent you choose, the analytical guidelines for the ABI 7000 fluorescence PCR instrument can save time and provide reliable results. Different target genes do not need to optimize the amplification system and parameters separately, and the basic components of analysis guidance include:

• Primer Express® software provides automatic primer and probe design

• TaqMan® and SYBR® Green I Master Mixes simplify PCR loading

• Multiple temperature cycle parameters allow you to perform different sample tests in the same sample plate

• Default primer and probe concentration settings do not require analysis optimization